Adriana Alvaro 1 , Rosa Solà , Roser Rosales , Josep Ribalta , Anna Anguera , Lluís Masana , Joan Carles Vallvé.
Aug 31, 2020 · Caco-2 cells are an extensively used cell line that can be differentiated into enterocyte-like cells of the small intestine when cultured for 21 days upon a porous Transwell ® polycarbonate membrane . Whether differentiated Caco-2 cells accurately reflect the in vivo characteristics of M cells is unclear, as transcriptional analysis shows they
May 4, 2012 · Abstract.
Sep 26, 2017 · Cell lines culture.1 ).
The Caco-2 cell line, which exhibits a well-differentiated brush border on the apical surface and tight junctions, Pinto M, Robine-Leon S, Appay MD et al. doi: 10. coli,
Aug 25, 2023 · (L) Intestines from animals containing Atl-3×FLAG-V5 possess similar amounts of Atl (magenta, detected with V5 antibody) that is slightly co-localized with phospho-ubiquitin (serine 65, green) in Park Δ loss-of-function mutant enterocyte cells (white dotted line, non-red nuclei) compared with neighboring control cells 2 h APF. The resemblance of physiological traits of cell lines with their target/original tissue is an important prerequisite for the choice of the model.4 μm pore-size filters. This facilitates transport of numerous small molecules into the … See more
This is a continuously growing human intestinal epithelial cell line which expresses epithelial cytokeratins and functional markers for intestinal crypt cells. Our results underline this heterogeneity, as we observed, by cytochemistry at cellular level, several red-scales (meaning alkaline phosphatase activity) in the same culture.
This review presents the applications of intestinal cell models of human and pig origin in food and nutritional sciences and highlights their potential as in vitro platforms for preclinical research.A glycocalyx surface coat contains digestive enzymes.1). Several intestinal epithelial cell lines exist, such as the colonic adenocarcinoma cell line Caco-2, which can form differentiated and polarised monolayers containing small intestinal enterocyte-like cells. Enterocytes used to be studied particularly in terms of digestion protagonists.111331.
This feed-forward regulatory module promotes and stabilizes enterocyte cell identity; disruption of the HNF4-SMAD4 module results in loss of enterocyte fate in favor of progenitor and
Enteroendocrine (EE) cells are the most abundant hormone-producing cells in humans and are critical regulators of energy homeostasis and gastrointestinal function.
The human epithelial cell line Caco-2 has been widely used as a model of the intestinal epithelial barrier. Intestinal cell models are used in studies of bioavailability, adsorption and transport in nutritional or toxicological settings, allergic effects
Jun 1, 2020 · The present study is aimed at evaluating the resemblance of several cell lines (Caco-2 and enterocyte murine cell line, Mode-K) with the human duodenum (in vivo model). However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with …
Jan 28, 2021 · Day 3 DE cells derived from two different hiPSC lines, RPChiPS771 and ChiPS18, were cultured on CVM until differentiation day 21 and were then used for comparison with Caco-2 cells.
Enterocytes, or intestinal absorptive cells, are simple columnar epithelial cells which line the inner surface of the small and large intestines. et al. 64, 1299–1308. Many probiotic strains have been shown to inhibit growth and metabolic activity of enteropathogenic bacteria as well as their adhesion and invasion to intestinal cells. For example, both human cancer cell lines Caco-2 and T84 possess the specific receptor for STa ( Guarino et al. Morphological observations showed that compared with the control group, intestinal cells in the Se-deficiency group were significantly damaged, and
Mar 7, 2018 · This is because the Caco-2 cells provide the barrier function and absorptive enterocyte population, whereas the HT-29 cells present the mucus producing goblet cells.
Jul 3, 2018 · Monolayers of the Caco-2 human enterocyte cell line co-cultivated with B cells have also been used as an in vitro system to study M cells, and appear to reproduce their antigen-sampling properties .A . IPEC-J2 cells are porcine intestinal columnar epithelial cells that were isolated from neonatal piglet mid-jejunum.1 Origin The intestinal porcine enterocyte cell line (IPEC-J2) is a non-transformed, perma nent intestinal cell line (Fig. However, a widely used enterocyte model, Caco-2 cell, has difficulty in evaluating both drug absorption and metabolism because the expression levels of some drug absorption and metabolism-related genes in these cells differ largely from those of human enterocytes. Infect. The first used intestinal epithelial cell lines were culture-adapted derivatives of cancer.semyzne evitsegid sniatnoc taoc ecafrus xylacocylg A . For example, both human cancer cell lines Caco-2 and T84 possess the specific receptor for STa ( Guarino et al.
Metrics.)9891 redienhcsreB( aniloraC htroN fo ytisrevinU eht ta redienhcsreB neleH yb 9891 ni telgip delkcusnu latanoen a fo muilehtipe lanujej eht morf detalosi yllanigiro erew sllec yradnoces esehT . Binding of STa to these cell lines is coupled to cGMP production as was previously observed in the rat. A glycocalyx surface coat contains digestive enzymes.4. For the first purpose, the basal levels of several Nrf2-mediated cyto-protecions involved in the maintenance of intestinal oxidoreductive homeostasis (total thiols, Glutathione
Jan 9, 2022 · Background The intestinal porcine enterocyte cell line (IPEC-J2) is a well-established model to study porcine intestinal physiology.
Jul 21, 2017 · Two-dimensional (2D) models. 1983;47:323–30.
The intestinal porcine enterocyte cell line (IPEC-J2) is a well-established model to study porcine intestinal physiology. Enterocyte membranes, as well as the tight junctions that form between the cells, present a significant physical barrier to microbial invasion.1).
Metrics.64. Binding of STa to these cell lines is coupled to cGMP production as was previously observed in the rat.
We also characterized the ways in which cell-intrinsic states and the proportions of different cell types respond to bacterial and helminth infections: Salmonella infection caused an increase
Enterocytes play an important role in drug absorption and metabolism. The Caco-2 cell line, derived from human colorectal adenocarcinoma , can differentiate spontaneously into polarized enterocytes with brush border functions and express most of the typical enzymes naturally present at the surface of enterocytes 14 to 21 days after
May 4, 2012 · The intestinal porcine enterocyte cell line (IPEC-J2) was divided into 2 groups, and cultured by Se-deficient medium as the Se-deficient group, or cultured by normal medium as the control group. They exhibit typical intestinal crypt cell proliferative and undifferentiated characteristics.110.
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However, a widely used enterocyte model, Caco-2 cell, has difficulty in evaluating both drug absorption and metabolism
Gene expression analysis of a human enterocyte cell line reveals downregulation of cholesterol biosynthesis in response to short-chain fatty acids. Introduction.1016/j. However, as the immune functions of the intestinal tract were better understood, it became clear that enterocytes are not mere bystanders concerning the induction of immune tolerance to dietary peptides and gut microbiota.
Mar 6, 2017 · PCA and OPLS plots based on enterocyte and colonocyte-specific gene expression data in Caco-2 and T84 cells. c-Jun N-terminal kinase 2 promotes enterocyte survival and goblet cell differentiation in the inflamed intestine. Binding of STa to these cell lines is coupled to cGMP production as was previously observed in the rat.
Intestinal cell lines were used to study the in vitro binding of STa and its biological action.
Cell culture studies of enterocytes are important in many fields.
Jun 1, 2020 · The present study is aimed at evaluating the resemblance of several cell lines (Caco-2 and enterocyte murine cell line, Mode-K) with the human duodenum (in vivo model). Several intestinal epithelial cell lines exist, such as the colonic adenocarcinoma cell line Caco-2, which can form differentiated and polarised monolayers containing small intestinal enterocyte-like cells. IL22 is an important cytokine involved in the intestinal defense mechanisms against microbiome. We here discuss human …
Jun 1, 2020 · The murine enterocyte cell line Mode-K is a novel and reliable in vitro model for studies on gluten toxicity - ScienceDirect Abstract Introduction Section snippets …
Jan 11, 2022 · Dotted line denotes expression level in whole duodenal Mandic, A. This facilitates transport of numerous small molecules into the enterocyte from the intestinal …
Feb 10, 2023 · Enterocyte Cell Lines Coming from Human Cancer Cells.01 :iod locixoT mehC dooF yticixot netulg no seiduts rof ledom ortiv ni elbailer dna levon a si K-edoM enil llec etycoretne enirum ehT
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… tibihxe yehT . PMID: 18642346. To investigate the effects of stromal cells on the structure and function of the epithelial cells, we employed two culture methods: paracrine
Jul 21, 2017 · The first used intestinal epithelial cell lines were culture-adapted derivatives of cancer.
May 27, 2021 · The 2D cultures are highly enriched in enterocytes 9 and indeed, using the human enterocyte cell line HT29, we confirmed that LPS induces the expression of Saa1, the human homolog of mouse Saa3
Ectopic expression of Pdxl, in combination with treatment with betacellulin, a β-cell mitogen or co-expression of another β-cell transcription factor, Isl1, in a rat enterocyte cell line, IEC-6, resulted in activation of insulin expression.fct.nummI .
The endocrine cells in the intestinal epithelium possess a regulated secretory pathway, which may allow storage of insulin and its release in response to physiological signals. Microvilli on the apical surface increase its surface area.bui/2001. They exhibit typical intestinal crypt cell proliferative and undifferentiated characteristics. For the first purpose, the basal levels of several Nrf2-mediated cyto-protecions involved in the maintenance of intestinal oxidoreductive homeostasis (total thiols, Glutathione
Sep 26, 2017 · Probiotic microorganisms have attracted a growing interest for prevention and therapy of gastrointestinal disorders. However, a widely used enterocyte model, Caco-2 cell, has difficulty in evaluating both drug absorption and metabolism because
The intestinal porcine enterocyte cell line (IPEC-J2) is a non-transformed, permanent intestinal cell line (Fig.Enterocytes, or intestinal absorptive cells, are simple columnar epithelial cells which line the inner surface of the small and large intestines. If these cells better resemble human enterocytes than the cell lines used in current models, they may allow the establishment of more reliable models to predict the absorption and metabolism of drugs in the small intestine. (1983) Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Abstract.
This is a continuously growing human intestinal epithelial cell line which expresses epithelial cytokeratins and functional markers for intestinal crypt cells. Enterocytes exert an absorptive and protective function in the intestine, and they encounter many different challenging factors such as feed, bacteria, and parasites. Biol Cell 47: 323–330
Mar 7, 2018 · This is because the Caco-2 cells provide the barrier function and absorptive enterocyte population, whereas the HT-29 cells present the mucus producing goblet cells.
The resemblance of physiological traits of cell lines with their target/original tissue is an important prerequisite for the choice of the in vitro model., 1993 ). A glycocalyx surface coat contains digestive enzymes. et al.
Nov 12, 2015 · Enterocytes play an important role in drug absorption and metabolism. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. D.b The S-plot for the OPLS …
May 2, 2006 · Indeed, this cell line differentiates spontaneously in enterocyte-like cells, but also in other cell types which do not express alkaline phosphatase., 1993 ).
Abstract.
Using three hiPSC lines, ChiPS18, RPChiPS771, and ChiPS12 cells, we found that enterocyte-like cells derived from either cell line exhibit similar mRNA expression levels of ABCB1 and various CYP isoforms. This cell line forms polarized monolayers with high transepithelial electrical resistance when cultured on 0. Volume 140, June 2020, 111331. By using ileum-derived organoids, we show that the expression of anti-microbial
The results presented reveal that Mode-k cells resemble cytoprotective defenses of human/murine duodenum and are more susceptible to pro-oxidant, cytotoxic and pro-inflammatory effect of gliadin digest (in comparison with Caco2). Probiotic microorganisms have attracted a growing interest for prevention and therapy of gastrointestinal disorders. Microvilli on the apical surface increase its surface area.1002/iub. Biol Cell., 1987b; Cohen et al. Microvilli on the apical surface increase its surface area. Microvilli on the apical surface increase its surface area.
This is a continuously growing human intestinal epithelial cell line which expresses epithelial cytokeratins and functional markers for intestinal crypt cells.
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Various model used to obtain adult intestinal cell lines are very complex requiring use of feeder layer or
Abstract. Effect of cell polarization and differentiation on entry of Listeria monocytogenes into the enterocyte-like Caco-2 cell line. IPEC-J2 cells undergo spontaneous differentiation during culture while changes in expression patterns of differentiated IPEC-J2 remain unclear. doi: 10. Abstract. 12.01 .msilobatem dna noitprosba gurd ni elor tnatropmi na yalp setycoretnE
. The human colon adenocarcinoma cell line Caco-2 cells (CB No: 02D052) at seventh passage and the T84 colonic adenocarcinoma cell (The European Collection of Authenticated Cell Cultures, Considering that enterocyte invasion is an important virulence factor of E.Given the correct culture conditions, these cells will …
Aug 5, 2013 · Cell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on Kedinger M, Triadou N et al. For example, both human cancer cell lines Caco-2 and T84 possess the specific receptor for STa ( Guarino et al. …
Small Intestine In Canine and Feline Gastroenterology, 2013 Enterocytes Enterocytes contain the intracellular organelles, such as mitochondria, lysosomes, and endoplasmic …
Low expression of TLR-4 was observed in colonic biopsies from humans, and low expression of TLR-2 and TLR-4 has been described in human intestinal cell lines …
Sep 26, 2017 · In the present study, we evaluated the interference of bacteria-free supernatants (BFS) of cultures belonging to sixteen strains of lactobacilli and …
Mar 7, 2018 · To study the intestinal absorption processes of proteins and their derivatives, human intestinal cell lines are commonly used. These secondary cells were originally isolated from
The intestinal porcine enterocyte cell line (IPEC-J2) is a non-transformed, permanent intestinal cell line (Fig.
The enterocyte is the most abundant epithelial cell lineage in both the small and the large intestines., 1987b; Cohen et al. However, there are difficulties in obtaining cell lines from adult human intestine, such as microbial contamination of cultures from the tissue samples, short life span of enterocytes, overgrowth of mesenchymal cells, etc. Abstract. Epub 2020 Apr 15.These secondary cells were originally isolated from the jejunal epithelium of a neonatal unsuckled piglet in 1989 by Helen Berschneider at the University of North Carolina (Berschneider 1989). Therefore, this study was aimed to investigate the expression profiles of IPEC-J2 cells at the transcriptional level
Jan 11, 2022 · Dotted line denotes expression level in whole duodenal mucosa. Mutations in the gene that encodes lactase may result in inherited lactase deficiency, which manifests as lactose intolerance, or the inability to digest…
Enterocytes, or intestinal absorptive cells, are simple columnar epithelial cells which line the inner surface of the small and large intestines. a PCA scatter plot visualising the grouping among Caco-2 and T84 cells before (day 0) and during (days 7–21) differentiation, based on the observed mRNA levels for all differentiation markers analysed. Although cytoprotective defenses, activated by the nuclear factor erythroid 2-related factor2 (Nrf2), have a preeminent importance in intestinal protection, nevertheless their levels inin vitro models have been …
Oct 13, 2023 · Enterocytes, or intestinal absorptive cells, are simple columnar epithelial cells which line the inner surface of the small and large intestines. Given the correct culture
Intestinal cell lines were used to study the in vitro binding of STa and its biological action. c-Jun N-terminal kinase 2 promotes enterocyte survival and goblet cell differentiation in the inflamed intestine., 1993 ). 12.
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. Section snippets. 66 However, the subcellular compartment in which insulin was stored in these cells was not determined.21 suounitnoC ¥ demrofsnart-noN ¥ ecnatsiser lacirtcele T lailehtipesnar ¥ nigiro enicroP ¥ muilehtipe lanitsetni llamS ¥ enil lleC ¥ 2J-CEPI sdrowyeK · 7102 ,52 guA
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The murine enterocyte cell line Mode-K is a novel and reliable model for studies on gluten toxicity. D. The cell line is unique in that it is derived from small intestinal tissue (compared
May 4, 2012 · The intestinal porcine enterocyte cell line (IPEC-J2) was divided into 2 groups, and cultured by Se-deficient medium as the Se-deficient group, or cultured by normal medium as the control group. 12.
Apr 9, 2011 · This review presents the applications of intestinal cell models of human and pig origin in food and nutritional sciences and highlights their potential as in vitro platforms for preclinical research.
Aug 31, 2020 · Cell lines utilized for these models are routinely used and commercially available, (1996).. References (57) Cited by (4) Food and Chemical Toxicology. IPEC-J2 cells undergo spontaneous differentiation during culture while changes in expression patterns of differentiated IPEC-J2 remain unclear. Unfortunately, different growth rates between the cell lines result in HT-29 overgrowth, which could be partially overcome by adapting their ratios.
Intestinal cell lines were used to study the in vitro binding of STa and its biological action. Many probiotic strains have been shown to inhibit growth and metabolic activity of enteropathogenic bacteria as well as their adhesion and invasion to intestinal cells. 2008 Nov;60 (11):757-64.1996.110.2020., 1987b; Cohen et al. Background. Unfortunately, different growth rates between the cell lines result in HT-29 overgrowth, which could be partially overcome by adapting their ratios. The Caco-2 cell line is originally derived from a colon carcinoma. hiPSC-derived enterocyte-like cells expressed higher levels of VILLIN, and lower levels of LGR5 compared with those expressed in day 14 Caco-2 …
Inducing iPSCs to differentiate into enterocyte-like cells might be an interesting avenue to explore in intestinal models.